An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis.
Identifieur interne : 002163 ( Main/Exploration ); précédent : 002162; suivant : 002164An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis.
Auteurs : S. Wongkamchai [Thaïlande] ; W. Satimai [Thaïlande] ; S. Loymek [Thaïlande] ; H. Nochot [Thaïlande] ; J J Boitano [Thaïlande]Source :
- Journal of helminthology [ 1475-2697 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Brugia malayi (immunologie), Brugia malayi (isolement et purification), Enfant, Enfant d'âge préscolaire, Femelle, Filariose lymphatique (diagnostic), Filariose lymphatique (parasitologie), Filariose lymphatique (sang), Humains, Immunoglobuline G (sang), Mâle, Sensibilité et spécificité, Test ELISA ().
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Brugia malayi.
- isolement et purification : Brugia malayi.
- parasitologie : Filariose lymphatique.
- sang : Anticorps antihelminthe, Filariose lymphatique, Immunoglobuline G.
- Animaux, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Mâle, Sensibilité et spécificité, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Brugia malayi (immunology), Brugia malayi (isolation & purification), Child, Child, Preschool, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay (methods), Female, Humans, Immunoglobulin G (blood), Male, Sensitivity and Specificity.
- MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- blood : Elephantiasis, Filarial.
- diagnosis : Elephantiasis, Filarial.
- immunology : Brugia malayi.
- isolation & purification : Brugia malayi.
- methods : Enzyme-Linked Immunosorbent Assay.
- parasitology : Elephantiasis, Filarial.
- Animals, Child, Child, Preschool, Female, Humans, Male, Sensitivity and Specificity.
Abstract
The aim of this study was to develop a low-cost antifilarial immunoglobulin (Ig) G4 detection kit for the diagnosis of lymphatic filariasis. The kit was designed to be used by minimally trained personnel without the constraints of expensive laboratory equipment. We provide a description of the development and validation of a single-serum-dilution based enzyme-linked immunosorbent assay (ELISA) kit with ready-to-use reagents for measuring antifilarial IgG4 antibodies. The kit was tested on residents in Brugia malayi-endemic areas in southern Thailand. Detection was performed by naked-eye observation of the resultant colour of the immunological reactivity. The coefficient of variation (CV) was used to assess the reproducibility of the results. Long-term stability was measured over a 6-month period. Sensitivity of the test kit was 97% when compared with microfilariae detection in thick blood smears. Specificity was 98.7% based on the sera of 57 patients living outside the endemic areas who were infected with other parasites and 100 parasite-free subjects. All positive CVs were < 10%. The test kit was remarkably stable over 6 months. Field validation was performed by the detection of antifilarial IgG4 in 4365 serum samples collected from residents of brugian filariasis-endemic areas and compared with outcome colours of the test samples by the naked eye. Subsequent ELISA evaluation of these results using an ELISA reader indicated high agreement by the kappa statistic. These results demonstrate that the test kit is efficient and useful for public health laboratories as an alternative tool for the diagnosis of lymphatic filarial infection.
DOI: 10.1017/S0022149X14000522
PubMed: 24916386
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001492
- to stream PubMed, to step Curation: 001492
- to stream PubMed, to step Checkpoint: 001492
- to stream Ncbi, to step Merge: 006744
- to stream Ncbi, to step Curation: 006744
- to stream Ncbi, to step Checkpoint: 006744
- to stream Main, to step Merge: 002166
- to stream Main, to step Curation: 002163
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis.</title><author><name sortKey="Wongkamchai, S" sort="Wongkamchai, S" uniqKey="Wongkamchai S" first="S" last="Wongkamchai">S. Wongkamchai</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Satimai, W" sort="Satimai, W" uniqKey="Satimai W" first="W" last="Satimai">W. Satimai</name><affiliation wicri:level="1"><nlm:affiliation>Bureau of Vector-Borne Disease, Department of Disease Control,Nonthaburi Province,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Bureau of Vector-Borne Disease, Department of Disease Control,Nonthaburi Province</wicri:regionArea><wicri:noRegion>Nonthaburi Province</wicri:noRegion></affiliation></author><author><name sortKey="Loymek, S" sort="Loymek, S" uniqKey="Loymek S" first="S" last="Loymek">S. Loymek</name><affiliation wicri:level="1"><nlm:affiliation>Filaria Project, Phikulthong Royal Development Study Center,Narathiwatt,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Filaria Project, Phikulthong Royal Development Study Center,Narathiwatt</wicri:regionArea><wicri:noRegion>Narathiwatt</wicri:noRegion></affiliation></author><author><name sortKey="Nochot, H" sort="Nochot, H" uniqKey="Nochot H" first="H" last="Nochot">H. Nochot</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Boitano, J J" sort="Boitano, J J" uniqKey="Boitano J" first="J J" last="Boitano">J J Boitano</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:24916386</idno><idno type="pmid">24916386</idno><idno type="doi">10.1017/S0022149X14000522</idno><idno type="wicri:Area/PubMed/Corpus">001492</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001492</idno><idno type="wicri:Area/PubMed/Curation">001492</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001492</idno><idno type="wicri:Area/PubMed/Checkpoint">001492</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001492</idno><idno type="wicri:Area/Ncbi/Merge">006744</idno><idno type="wicri:Area/Ncbi/Curation">006744</idno><idno type="wicri:Area/Ncbi/Checkpoint">006744</idno><idno type="wicri:Area/Main/Merge">002166</idno><idno type="wicri:Area/Main/Curation">002163</idno><idno type="wicri:Area/Main/Exploration">002163</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis.</title><author><name sortKey="Wongkamchai, S" sort="Wongkamchai, S" uniqKey="Wongkamchai S" first="S" last="Wongkamchai">S. Wongkamchai</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Satimai, W" sort="Satimai, W" uniqKey="Satimai W" first="W" last="Satimai">W. Satimai</name><affiliation wicri:level="1"><nlm:affiliation>Bureau of Vector-Borne Disease, Department of Disease Control,Nonthaburi Province,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Bureau of Vector-Borne Disease, Department of Disease Control,Nonthaburi Province</wicri:regionArea><wicri:noRegion>Nonthaburi Province</wicri:noRegion></affiliation></author><author><name sortKey="Loymek, S" sort="Loymek, S" uniqKey="Loymek S" first="S" last="Loymek">S. Loymek</name><affiliation wicri:level="1"><nlm:affiliation>Filaria Project, Phikulthong Royal Development Study Center,Narathiwatt,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Filaria Project, Phikulthong Royal Development Study Center,Narathiwatt</wicri:regionArea><wicri:noRegion>Narathiwatt</wicri:noRegion></affiliation></author><author><name sortKey="Nochot, H" sort="Nochot, H" uniqKey="Nochot H" first="H" last="Nochot">H. Nochot</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Boitano, J J" sort="Boitano, J J" uniqKey="Boitano J" first="J J" last="Boitano">J J Boitano</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok,Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Parasitology,Faculty of Medicine, Siriraj Hospital, Mahidol University,Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of helminthology</title><idno type="eISSN">1475-2697</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Brugia malayi (immunology)</term><term>Brugia malayi (isolation & purification)</term><term>Child</term><term>Child, Preschool</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Female</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Male</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Brugia malayi (immunologie)</term><term>Brugia malayi (isolement et purification)</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (parasitologie)</term><term>Filariose lymphatique (sang)</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Mâle</term><term>Sensibilité et spécificité</term><term>Test ELISA ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Filariose lymphatique</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Male</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Sensibilité et spécificité</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to develop a low-cost antifilarial immunoglobulin (Ig) G4 detection kit for the diagnosis of lymphatic filariasis. The kit was designed to be used by minimally trained personnel without the constraints of expensive laboratory equipment. We provide a description of the development and validation of a single-serum-dilution based enzyme-linked immunosorbent assay (ELISA) kit with ready-to-use reagents for measuring antifilarial IgG4 antibodies. The kit was tested on residents in Brugia malayi-endemic areas in southern Thailand. Detection was performed by naked-eye observation of the resultant colour of the immunological reactivity. The coefficient of variation (CV) was used to assess the reproducibility of the results. Long-term stability was measured over a 6-month period. Sensitivity of the test kit was 97% when compared with microfilariae detection in thick blood smears. Specificity was 98.7% based on the sera of 57 patients living outside the endemic areas who were infected with other parasites and 100 parasite-free subjects. All positive CVs were < 10%. The test kit was remarkably stable over 6 months. Field validation was performed by the detection of antifilarial IgG4 in 4365 serum samples collected from residents of brugian filariasis-endemic areas and compared with outcome colours of the test samples by the naked eye. Subsequent ELISA evaluation of these results using an ELISA reader indicated high agreement by the kappa statistic. These results demonstrate that the test kit is efficient and useful for public health laboratories as an alternative tool for the diagnosis of lymphatic filarial infection.</div></front></TEI><affiliations><list><country><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Wongkamchai, S" sort="Wongkamchai, S" uniqKey="Wongkamchai S" first="S" last="Wongkamchai">S. Wongkamchai</name></noRegion><name sortKey="Boitano, J J" sort="Boitano, J J" uniqKey="Boitano J" first="J J" last="Boitano">J J Boitano</name><name sortKey="Loymek, S" sort="Loymek, S" uniqKey="Loymek S" first="S" last="Loymek">S. Loymek</name><name sortKey="Nochot, H" sort="Nochot, H" uniqKey="Nochot H" first="H" last="Nochot">H. Nochot</name><name sortKey="Satimai, W" sort="Satimai, W" uniqKey="Satimai W" first="W" last="Satimai">W. Satimai</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002163 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002163 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24916386
|texte= An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24916386" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |